Xenbase Image ID: 156527

Figure 4. DNAJB6-like Proteins Reduce Toxicity and Suppress Aggregation In Vivo (A) HEK293 cells were cotransfected with HDQ119-EYFP and chaperones as indicated. The percentage of propidium iodide (PI)-positive cells was measured in the YFP-positive fraction; data are presented as mean ±SEM (N = 2). (B) Same as in (A) for SHSY-5Y cells. (C and D) Clonogenic assay of HEK293 cells stably expressing tetracycline-inducible HDQ119-EYFP with or without constitutive DNAJB8 expression. DNAJB8 expression alone did not alter the clonogenicity (inset). The number of clones obtained in the absence of HDQ119-EYFP expression was set to 100%; data are presented as mean ±SD (N = 3). (E) Transgenic HDQ119-EYFP-positive tadpoles expressing the indicated chaperones analyzed by fluorescence microscopy (F). Lower panels show bright-field images (BF). (F) Close-up of muscle fibers from (E). (G) Percentage of aggregate-containing transgenic tadpoles with or without the expression of DNAJB chaperones. Number of investigated transgenic tadpoles is indicated. Image published in: Hageman J et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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