Xenbase Image ID: 156529

Figure 6. The Importance of the C-Terminal Domain of DNAJB8 for Activity, Substrate Binding, and Oligomerization (A–C) Schematic representation of systematic DNAJB8 deletion mutants (A) and their effect on HDQ119-EYFP aggregation (B and C) measured as in Figure 1. (D) Cells transfected with YFP-HDQ119 and CFP-tagged DNAJB8 wild-type and mutant variants as indicated were analyzed for fluorescence resonance energy transfer (FRET) using fluorescence lifetime (FLIM) analysis; data are mean ±SD (N > 9 individual cells). (E) Sucrose density gradient fractionation of lysates of cells expressing DNAJB8 wild-type and mutant variants as indicated. DNAJB8 and its mutants were detected using anti-V5 antibody, whereas endogenous DNAJB1 and HSPA1A/B were detected with DNAJB1- or HSPA1A/B-specific antibodies. Image published in: Hageman J et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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