Xenbase Image ID: 156555

Fig. 5. Photomicrographs of doubly labeled transverse sections through the Xenopus developing forebrain showing the x-Shh expression at premetamorphic stages in combination with NKX2.1 (a, e, f, g, j), TH (b–d, k–m) and ISLET1 (n). At preoptic regions, the double x-Shh/NKX2.1 labeling confirmed the x-Shh telencephalic expression in the preoptic commissural area (a), whereas the double x-Shh/TH staining delineates the preoptic area (b, c). Similarly, x-Shh/TH (d) and x-Shh/NKX2.1 (g) allowed the identification of the SC, rich in TH and NKX2.1 expressing cells (see arrowhead in g). In the diencephalon, the x-Shh expressing cells in the Zli extend into the prethalamic (P3) territory, showing NKX2.1 expression (arrowheads in e and f). In posterior diencephalic levels, the x-Shh detected was situated in P3 by the double x-Shh/NKX2.1 (h–j), and specifically in the zona incerta, according to the TH expressing cells detected (k). The double labeling for x-Shh/TH (l, m) and x-Shh/ISLET1 (n) allowed the identification of the mammillary and tuberal boundaries. Scale bars = 100 μm (a–n) and 50 μm (b′). Image published in: Domínguez L et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue shh.L hedgehog, vhh-1, X-SHH, Xhh, xshh X. laevis Sometime during NF stage 20 to NF stage 35 and 36   nkx2-1.L nkx2.1, titf1, ttf1, Xnkx-2.1, xnkx2-1 X. laevis Sometime during NF stage 13 to NF stage 35 and 36   Image source: Published Larger Image Printer Friendly View

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