XB-IMG-156653
Xenbase Image ID: 156653
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Fig. 4. Immuno-electron micrographs of a saline-washed RPE-eyecup. Light-adapted adult Xenopus were killed at midmorning,
and detached RPE-eyecups were washed with saline. Sections were incubated with anti-Xenopus IRBP serum followed
by protein A-labeled 10 nm colloidal gold. In contrast to the unwashed retina (Fig. 3), the extracellular region (asterisks) is
devoid of gold label. IRBP was not detected coating the extracellular surface of the RPE or its microvilli (arrowheads). (A) and
(B) IRBP contained within vesicles (v) in the apical RPE cytoplasm. These structures are shown at higher magni®cation in (C)
and (D). The gold label is often localized to the inner surface of the vesicle (open arrows). Sections treated with pre-immune
serum showed no speci®c labeling (data not illustrated). Bar¯0±5 lm in (A) and 1±0 lm in (B), (C), (D). Image published in: Cunningham LL et al. (1999) Copyright © 1999. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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