XB-IMG-156654
Xenbase Image ID: 156654
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Fig. 5. Specific activities of IRBP and opsins following systemic injection of [$&S]methionine. Juvenile Xenopus were injected
in the dorsal lymph sac with 500 lCi of [$&S]methionine. The speci®c activities of IRBP and opsin were measured at various
timepoints following injection. (A) Representative Coomassie stained SDS polyacrylamide gels of the soluble matrix (Matrix,
arrow indicates IRBP) and the retinal membrane fraction (Membranes, bracket indicates opsin). (B) Representative
PhosphorImager bands ($&S) for both IRBP (arrow) and opsin (bracket) as well as the corresponding Coomassie blue-stained
band (CB) from the same eye. Time (days post-injection) is indicated at the top of the panel. (C) Specific activities of IRBP and
opsin as a function of time following injection. IRBP speci®c activity (E) remains constant, while opsin specific activity (+)
increases over time. Image published in: Cunningham LL et al. (1999) Copyright © 1999. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |