Xenbase Image ID: 156702

Figure 1. Isolation of Genes that Activate the Expression of Posterior Region–Specific Genes (A) Expression screening strategy. A gastrula expression library was divided into 100 fractions of 1000 clones each, and mRNA was synthesized from each pool by in vitro transcription. The synthetic RNA was injected into animal poles of two-cell stage embryos, and ectodermal explants were isolated at blastula stages. The positive pools were identified by analyzing the expression of posterior-specific genes at neurula stages by RT-PCR. (B) Differing amounts of 20CHG11 RNA (0–5 ng) were injected into animal poles of two-cell embryos (lanes 3–6). Ectodermal explants were isolated at blastula stages and subjected to RT-PCR analysis at neurula stages (stage 15). −RT indicates sibling control embryos processed without reverse transcriptase. Histone was used as a loading control. (C) 20CHG11 induces the ventral mesodermal marker, Xhox3, but not other regional markers such as Xwnt-8 and chordin, whose expression is localized to the ventral and dorsal sides of embryo, respectively. Ectodermal explants injected with 20CHG11 RNA were processed for RT-PCR assay at early gastrula stages (stage 11.5). Image published in: Suzuki A and Hemmati-Brivanlou A (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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