Xenbase Image ID: 156707

Figure 6. xE2F:EnR Acts as a Dominant-Negative xE2F (A) Schematic representation of the xE2F:EnR construct. The DNA-binding domain (DBD) of wild-type xE2F is indicated as a black box. A dark gray box indicates the transactivation domain (TAD). Light gray boxes indicate the repressor domain from the Drosophila Engrailed gene. (B) xE2F:EnR suppresses the expression of HoxB9 induced by wild-type xE2F in ectoderm. xE2F RNA (0.3 ng) was injected with increasing amounts of xE2F:EnR RNA (30–125 pg) into the animal poles of two-cell embryos (lanes 5–7). The explants were isolated at blastula stages, and the expression of HoxB9 was detected by RT-PCR at neurula stages. Overexpression of the EnR domain alone does not affect the expression of HoxB9 (lane 9). (C) Suppression of xE2F function by xE2F:EnR (dnxE2F) is rescued by increasing amounts of xE2F. The induction of HoxB9 expression by 0.15 ng of xE2F RNA is suppressed by coinjection of 30 pg of dnxE2F. This suppression is rescued by injection of 0.6 ng xE2F RNA. Image published in: Suzuki A and Hemmati-Brivanlou A (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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