Xenbase Image ID: 156721

Figure 7. Functional and Structural Analysis of the Interaction between XSox17β and β-catenin (A) Second axis assay. Embryos were injected with the indicated doses of XSox17β and pt-β-catenin RNA, and the frequency of secondary axis was scored. (B) OT reporter assay. Human 293T cells were cotransfected with OT reporter construct containing TCF consensus binding sites (Korinek et al. 1997) and the indicated amounts of expression plasmids encoding S37A-β-catenin and XSox17β. (C and D) The indicated 35S-labeled Xβ-catenin deletion proteins were incubated with nickel-agarose resin or nickel-resin coupled with 1 μg of His-XSox17β protein (C). The relative binding of each deletion protein to His-XSox17β (based on three separate experiments) is indicated (D), and a representative experiment is shown. The minimal XSox17β binding site overlaps with the established TCF binding site. Image published in: Zorn AM et al. (1999) Copyright © 1999. Image reproduced with permission of the Publisher, Elsevier B. V. Experiment + Assay Source Phenotypes and Disease Xla Wt + ctnnb1tm1 + tadpole stage (morphology) fig.7.a Anatomical Phenotype obsolete duplicated anterior-posterior axis Xla Wt + ctnnb1tm1 + sox17b.2 + tadpole stage (morphology) fig.7.a Anatomical Phenotype obsolete duplicated anterior-posterior axis Xla Wt + ctnnb1tm1 + sox17b.2 + tadpole stage (morphology) fig.7.a Anatomical Phenotype obsolete duplicated anterior-posterior axis Larger Image Printer Friendly View

Return to previous page