XB-IMG-1568
Xenbase Image ID: 1568
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Fig. 6. Presenilin up-regulates shh and down-regulates chd in a Notch-dependent way. In situ hybridisation of (A-F) shh and (G-P) chd. Whole embryos in A-C,G-I,M,N are shown in dorsal views, anterior up. (A) Stage 14 embryo injected with 4.5 ng of nuc-lacZ mRNA (nucβgal). (B) Stage 14 embryo injected with 2 ng of X-ps-α . (C) Stage 14 embryo injected with 2 ng of X-ps-α plus 2 ng of X-su(H)DBM. (D) Transverse section of the same embryo shown in A. (E) Transverse section of the same embryo shown in B. Notice the expansion of the floor plate domain of shh on the injected side (arrow). (F) Transverse cut of a stage 16 embryo injected with 2 ng of X-ps-α plus 2 ng of X-su(H)DBM. Owing to the strong inhibition, the visualisation of shh expression in 50μ m vibratome sections from these embryos was difficult. In order to appreciate differences between the injected and non-injected side, we show here a transverse cut obtained with a scalpel. The asterisk indicates the repression of shh expression in the floor plate domain on the right side, where X-gal stained cells are preferentially located. Notice that the notochord is complementarily augmented on this side. Overall, the notochord is larger than in embryos of the same stage injected with equal amounts of nuc-lacZ mRNA (not shown). The broken line in D-F outlines the notochord. (G) Stage 13 embryo injected with 4 ng of nuc-lacZ mRNA. (H) Stage 13 embryo injected with 2 ng of X-ps-α. (I) Stage 13 embryo injected with 2 ng of X-ps-α plus 2 ng of X-su(H)DBM. (J,K,L) Transverse sections of the embryos shown in G, H and I respectively. (M) Stage 12.5 embryo injected with 5 ng of a control morpholino. (N) Stage 12.5 embryo injected with 5 ng of an antisense morpholino oligonucleotide against X-ps-α. (M,P) Transverse sections of the embryos shown in M and N respectively. All sections, except F, are Nomarski interference contrast views. Image published in: López SL et al. (2003) Copyright © 2003. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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