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Figure 1. PAP restores drought tolerance and ABA-responsive stomatal closure in ABA signaling mutants.(A) Representative photos of two plants per genotype subjected to 10 days of drought. Statistically significant differences in survival during drought between genotypes are indicated (n = 4 per genotype per experiment, three independent experiments). (B) Effect of 20 µM ABA on stomatal conductance (gs) after 2 hr feeding through the roots of hydroponically-grown plants. The data is the average of two independent experiments (n = 3 plants per genotype per experiment) ± SEM. (C) The effect of 50 µM ABA treatment for 2 hr on stomatal aperture of leaf peels from five to six-week old plants.DOI: http://dx.doi.org/10.7554/eLife.23361.002Figure 1—figure supplement 1. PAP content in single and double mutants; and SAL1 localization in epidermal peels and guard cell chloroplasts.(A) Quantification of PAP in well-watered (WW) plants shows PAP levels in double mutants (dark bars) were not significantly different to sal1-8 (light bar). Data are means ± SEM, n = 5. Significant differences (p<0.05) are indicated by a, b. n.d. = not detected. (B) Western blots of total leaf, veins and epidermal peels isolated from 51 day-old Col-0 plants showed SAL1 could be detected in epidermal peels, whereas mesophyll and bundle sheath localized proteins, PEPC and APX were barely detectable. (C) Leaves of adult plants expressing pSAL1:SAL1:GFP examined using a Leica confocal microscope showed GFP fluorescence in the chloroplasts of guard cells.DOI: http://dx.doi.org/10.7554/eLife.23361.003 Image published in: Pornsiriwong W et al. (2017) © 2017, Pornsiriwong et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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