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Figure 6. Exogenous PAP does not stimulate cytosolic Ca2+ transients in guard cells.Three representative Ca2+ oscillation patterns obtained from time-resolved Ca2+ imaging experiments with PAP treatment. Numbers of observed cells in each group are labeled above each graph (measurements were obtained from 10 different plants). Guard cells of YC3.6 plants were monitored for their FRET emission at 535 nm and 480 nm. FRET ratio increases after the addition of 10 mM Ca2+ suggesting the cells are capable of sensing intracellular Ca2+ level changes.DOI: http://dx.doi.org/10.7554/eLife.23361.014 Image published in: Pornsiriwong W et al. (2017) © 2017, Pornsiriwong et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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