Xenbase Image ID: 156830

Figure 7. Interaction between PAP-mediated signaling with Ca2+.(A) Wild type stomatal aperture with or without PAP in the presence of low (50 µM) Ca2+ or high (1 mM) Ca2+ in the measuring buffer. Means ± SEM for 9–10 stomata per treatment are shown. Significant differences between treatments at t = 60 min (ANOVA, p<0.05) are indicated by a, b. (B) Stomatal aperture in leaf peels treated with PAP and an intracellular calcium chelator (BAPTA-AM) or an extracellular calcium chelator (EGTA).Values are relative to control (measuring buffer). Means ± SEM for 18 stomata from four plants per treatment are shown. Significant differences between treatments at t = 60 min (ANOVA, p<0.05) are indicated by a, b. (C) Stomatal aperture in leaf peels treated with control (ethanol), 100 µM PAP or 10 µM ABA in the presence of low Ca2+ (50 µM). Means and SEM for four plants with >28 stomata per plant are shown. Significant differences between treatments (ANOVA, p<0.05) are indicated by a, b. (D) Stomatal aperture in leaf peels that were pretreated with 20 µM diphenyleneiodonium (DPI) prior to treatment with either 100 µM PAP or 100 µM ABA, in measuring buffer. The control treatment was leaf peels which were not pretreated with DPI and were treated with measuring buffer. Values are means ± SEM for 41–51 stomata per treatment. Final level of closure was considered by ANOVA after treatment; significant difference (p<0.05) denoted a, b.DOI: http://dx.doi.org/10.7554/eLife.23361.015 Image published in: Pornsiriwong W et al. (2017) © 2017, Pornsiriwong et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page