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FIG. 4. Both X. laevis Slug promoters drive specific expression in NCCs. Constructs containing the GFP coding region under the control of the slug or slug promoter were injected into one blastomere of a two-cell stage embryo, and GFP levels were monitored throughout early development. The dashed lines indicate the midline of the embryo. e, eye vesicle; c, cement gland. With both constructs, a pattern similar to that for endogenous Slug was obtained. Note that at stage (St.) 25 NF, migrating cells colonizing the branchial arches were still GFPpositive, but the signal was weaker because the acquisition time required to detect it was three times longer (inset in the upper right panel).

Image published in: Vallin J et al. (2001)

Copyright © 2001. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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