XB-IMG-156895
Xenbase Image ID: 156895
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FIG. 3. Effect of injected RNA probes on endogenous CamKII activity. Panel A, influence of pertussis toxin, kinase dead CamKII (CamKII
K42M), dominant negative Xwnt-11 (dnXwnt-11), and dominant negative Xwnt-8 (dnXwnt-8) RNA on Wnt induced CamKII activity. Embryos were
injected with RNAs and doses (ng) as indicated and the specific Ca21-independent CamKII activity was determined. n, number of experiments. The
increase in CamKII activity (above dotted baseline) upon injection of Xwnt-5A is sensitive to pertussis toxin, kinase dead CamKII, and dnXwnt-11
but not dnXwnt-8. Panel B, influence of constitutively active CamKII (CamKII T286D, 1 ng), kinase dead CamKII (CamKII K42M, 1–2 ng),
dnWnt-11 (1 ng), pertussis toxin A protomer (Ptx, 0.375 ng), and control prolactin (1 ng) RNA injections on endogenous CamKII activity. Embryos
were injected with RNAs at the 4-cell stage into both dorsal (upper panel) or both ventral blastomeres (lower panel). At stage 7 embryos were cut
into dorsal (M) and ventral (u) halves and immediately assayed for specific CamKII activity as described in the legend to Fig. 1. Endogenous levels
of CamKII activity in untreated dorsal (d) or ventral (v) halves of stage 7 embryos are given as comparison after normalization to protein content.
n 5 number of experiments. Panel C, dominant negative Xwnt-11 (dnWnt-11), but not dominant negative Xwnt-8 (dnWnt-8), can block Xwnt-5A
induced relocalization of PKC. Confocal image of stage 8 animal cap tissue from Xenopus embryos injected at the 2-cell stage into both cells with
XPKCa-myc (0.25 ng), Xwnt-5A (0.13 ng), prolactin (1 ng), dnXwnt-8 (1 ng), or dnXwnt-11 (1 ng) RNA as indicated. Detection of the c-myc epitope
to monitor PKC localization was as described (12). Image published in: Kühl M et al. (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |