Xenbase Image ID: 156896

FIG. 4. Dorso-ventral differences in endogenous CamKII activity in Xenopus embryos. Panel A, embryos were cut into ventral (u) and dorsal (M) halves at stage 7 and 10, respectively, and assayed for CamKII activity as described in the legend to Fig. 1. Ventral halves of stage 7 and stage 10 Xenopus embryos show a higher Ca21-independent CamKII activity than dorsal halves (left panel). Total kinase activity at stage 7, determined in the presence of Ca21, is the same in dorsal and ventral halves (right panel). Given CamKII activities were normalized to protein content. n 5 number of experiments. Panel B, spatial asymmetry in CamKII activity is accompanied by an enrichment of the autophosphorylated form of CamKII on the ventral side of the embryo at stage 7. Xenopus embryos were cut into dorsal (d) and ventral (v) halves before CamKII was immunoprecipitated with a CamKII-specific monoclonal antibody. Immunoprecipitates were blotted and probed with a polyclonal antibody recognizing the Thr286-phosphorylated form of CamKII (upper P-CamKII blot). Reprobing these blots with a CamKII specific antibody shows equal amounts of CamKII in dorsal and ventral halves (lower CamKII blot). Image published in: Kühl M et al. (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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