XB-IMG-156897
Xenbase Image ID: 156897
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FIG. 5. Effects of expressing CamKII mutants in Xenopus embryos.
Panel A, dorsal but not ventral injection of the constitutively
active mutant CamKII T286D (1 ng) results in ventralization of embryos
which is accompanied by loss of endogenous goosecoid (gsc) expression
at stage 10, and a loss of notochord formation at stage 30 (see
Table II). Ventral but not dorsal injection of the kinase dead mutant
CamKII K42M (1 ng) results in axis duplication at a low incidence
accompanied by a second domain of gsc expression at stage 10 (see
Tables II and III for details). Injection of prolactin (PL) control RNA (1
ng) had no effects on either axis formation or on gsc expression. Arrowheads
denote dorsal lips. Panel B, dorsal injection of the constitutively
active mutant results in up-regulation of ventral marker genes (compare
arrowheads of Xvent-2, Xvent-1, Xwnt-8, and GATA-2 relative to
arrowheads in control lacZ embryos) but not of the pan-mesodermal
marker Xbra in stage 10 embryos. Ventral injection of the kinase dead
mutant of CamKII results in decrease or loss of staining for ventral
markers but not of Xbra. lacZ injections had no effect. Image published in: Kühl M et al. (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |