XB-IMG-156903
Xenbase Image ID: 156903
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Fig. 2.
Neural patterning and differentiation are disrupted in sall morphants. Splice-blocking sall1 and sall4 morpholinos induce changes in neural marker gene expression, as assayed by whole-mount RNA in situ hybridization at stages 16 (A-T) and 23 (U-Y) using markers otx2, krox20, and hoxb9 in combination (A-E), pax3 (F-J), pax6 (K-O), sox2 (P-T), and tubb2b (U-Y); dorsal view, anterior up. Asterisks in images indicate injected side; arrows indicate partial loss of expression; arrowheads indicate posterior shifts in expression. Quantification (E, J, O, T, and Y) shows the percentage of embryos with symmetrical (white bars) or asymmetrical (grey bars) neural gene expression. These graphs include data from splice-blocking and translation-blocking morpholino experiments; see Supplementary Fig. 2 for translation-blocking morpholino images. n >30 embryos per condition; **, p<0.01, *** p<0.001 by pairwise chi-squared test. Expression of hoxb9 in morphants at earlier stages is shown in Supplementary Fig. 3. Image published in: Exner CRT et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V.
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