XB-IMG-156904
Xenbase Image ID: 156904
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Fig. 3.
Neural tube morphogenesis is disrupted in sall morphants. (A–D) Stage 16 embryos after unilateral injection of morpholino(s); dorsal view, anterior up. Asterisks in images indicate injected side; white arrowheads indicate normal neural fold formation; black arrowheads indicate failed neural fold formation. Quantification (E) shows the percentage of embryos with closing (white bars) or open (grey bars) neural tubes. n >500 embryos per condition; *** p<0.001 by pairwise chi-squared test. (F-I′) Confocal microscopy of transverse sections of morphants; dorsal is up. Sections were taken from the anterior third of embryos, where primary neurulation is most easily observed. F-actin (phalloidin, green), microtubules (anti-DM1α antibody, red), nuclei (DAPI, blue). Asterisks in images indicate injected side; white arrows indicate loss of actin accumulation. F′-I′ show higher magnification views of boxed regions in F-I. Scale bars =50 µm. s= somite, noto = notochord. Splice-blocking sall1 and sall4 morpholinos were used here (see Supplementary Fig. 7 for results with translation-blocking morpholinos). (J) Quantification, showing percentage of embryos with closing (lighter bars) or open (darker bars) neural tubes, further classified by actin laterality (see key). Graph includes data from splice-blocking and translation-blocking morpholino experiments. n >45 embryos per experimental condition; *** p<0.001 by pairwise chi-squared test.
Image published in: Exner CRT et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V.
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