Xenbase Image ID: 156905

Fig. 4. pou5f3 family gene expression is derepressed in sall morphants. Splice-blocking sall1 and sall4 morpholinos cause an increase in pou5f3 family gene expression, as assayed by whole-mount RNA in situ hybridization at stages 16 for pou25 (A-E), pou60 (F-J), and pou91 (K-O); dorsal view, anterior up. Asterisks in images indicate injected side. Quantification (E, J, and O) shows the percentage of embryos with symmetrical (white bars) or asymmetrical (grey bars) pou5f3 expression; n >16 embryos per condition, *** p<0.001 by pairwise chi-squared test. Quantitative RT-PCR data for bilaterally-injected sall morphants is shown in P-R; * p<0.05, ** p<0.01, *** p<0.001 by Tukey's Honestly Significantly Different test on data from 4 biological replicates. Image published in: Exner CRT et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue pou5f3.2.L Oct-25, oct25, pou25, xoct-25, xoct25 X. laevis Throughout NF stage 16 neural plate pou5f3.L Oct-91, oct4, oct79, oct91, oct92, pou5f3.1, XLPOU91, xoct-91, xoct91 X. laevis Throughout NF stage 16   pou5f3.3.L oct-60, oct60, pou60, XLPOU-60, XLPOU60, xoct60 X. laevis Throughout NF stage 16   Image source: Published Larger Image Printer Friendly View

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