Xenbase Image ID: 156907

Fig. 6. Neural tube morphogenesis is disrupted upon pou5f3 overexpression. (A–D) Stage 16 embryos after unilateral injection of mRNA; dorsal view, anterior up. Asterisks in images indicate injected side; white arrowheads indicate normal forming neural fold; black arrowheads indicate failed neural fold formation. Quantification (E) shows the percentage of embryos with closing (white bars) or open (grey bars) neural tubes. n >300 embryos per experimental condition; *** p<0.001 by pairwise chi-squared test. (F-I′) Confocal microscopy after transverse sectioning and staining of pou5f3-overexpressing embryos; dorsal is up. Sections were taken from the anterior third of embryos, where primary neurulation is most easily observed. F-actin (phalloidin, green), microtubules (anti-DM1α antibody, red), nuclei (DAPI, blue). Asterisks in images indicate injected side; white arrows indicate loss of actin accumulation. F′-I′ show higher magnification views of F-I. Scale bars =50 µm. s= somite, noto = notochord. Quantification (J) shows the percentage of embryos with closing (lighter bars) or open (darker bars) neural tubes, further classified by actin laterality (see key). n >55 embryos per experimental condition; *** p<0.001 by pairwise chi-squared test. Image published in: Exner CRT et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tuba4b.L alpha tubulin, alpha-tubulin, tuba4, tuba4a X. laevis Throughout NF stage 16 neural plate actb.L beta actin, beta-actin, g-actin X. laevis Throughout NF stage 16 neural plate Image source: Published Larger Image Printer Friendly View

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