Xenbase Image ID: 156913

Fig. 6. One Pax2 binding site is sufficient to activatealcampromoter activity in neural animal caps. A. A schematic drawing of different deletion constructs of the alcam promoter region. In the native promoter region, seven ATF2 binding sites (blue) and two Pax2 binding sites (yellow) were found. The different regulatory alcam promoter regions were cloned in front of the luciferase reporter gene. See Fig. S4 for more details. B. Promoter activity of different alcam promoter constructs in neuralized animal caps by co-injection of 600 pg noggin mRNA. C. Loss of Fzd3 led to a reduction of the promoter activity of −2.7kb-luc, whereas −2.7kb-∆ATF2/Pax2-luc was not sensitive to a reduction of Fzd3. D. Promoter activity of different promoter constructs as illustrated in A. in neuralized animal caps. Alcam promoter activity was lost in the absence of both Pax2 binding sites (blue bar). Alcam promoter activity was rescued by the presence of at least one Pax2 binding site (yellow bars). Abbreviations: n, number of independent experiment; n.s., not significant; RLU, relative light units. Error bars indicate standard error of the mean (s.e.m.); * p≤0.05. p values were calculated by a non-parametric Mann–Whitney rank sum test. Image published in: Seigfried FA et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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