XB-IMG-157113
Xenbase Image ID: 157113
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Fig. 12. Northern blot analysis of the expression of IRBP and
opsin during development. (A) Lanes A and B received 14 μg
each of total RNA extracted from stage 43 heads and bodies
respectively. Left panel: ethidium bromide-stained gel showing
molecular mass standards and ribosomal RNA before blotting.
Center panel: autoradiogram of blot probed with Xenopus IRBP
cDNA. Right panel: same blot stripped and reprobed with bovine
opsin cDNA. Lane S corresponds to the molecular mass standards.
(B) Each lane received 23 μg of total RNA extracted from whole
embryos. The developmental stage of the embryo is indicated
above the lane. Top panel: the blot was probed with antisense
Xenopus IRBP probe and exposed for 24 h. Bottom panel:the blot
was then stripped and reprobed with a Xenopus opsin (Saha and
Grainger, 1993) antisense probe and exposed for 6 h. Image published in: Gonzalez-Fernandez F et al. (1993) Copyright © 1993. Image reproduced with permission of the Publisher, The Company of Biologists Ltd.
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