XB-IMG-157139
Xenbase Image ID: 157139
Fig. 1.
eif6 interaction with the igf pathway. a. Co-immunoprecipitation using anti-eIF6 or anti-IGFR. The two isoforms of eif6 are immunoprecipitated by anti-IGFR and igfr is immunoprecipitated by anti-eIF6, in contrast to the control immunoprecipitation using IgG. b. Immunoblots of eif6-injected embryos or of GFP-injected embryos. Anti-AKT or anti-pAKT, as well as anti-pMAPK or anti-MAPK, were used: there is a clear decrease in pakt level in eif6-injected embryos compared to GFP-injected embryos. In contrast, in embryos injected with eif6 or with GFP no difference is observed between pmapk and mapK levels. Normalization was performed with anti-tubulin. c. Animal caps lysates of embryos injected with p110* of the PI3 kinase were analyzed. pAkt level is higher in embryos injected with p110* than in those injected with GFP. d.In situ hybridizations of neurula embryos using rax1 riboprobe. The hybridization by this marker specific for the eye indicates the extension of the eye field in the observed embryos. 1. Control embryos injected with GFP showing that the rax1 hybridization extension is practically identical in both sides of the embryo. 2. The side of the embryo injected with eif6 shows a rax1 hybridization less extended than the un-injected side. 3. In the embryo side co-injected with p110* and eif6 the eye field stained by rax1 is practically identical in both sides of the embryo indicating that a partial rescue of the eye field occurs in these embryos. Rescue means a partial or total recovery of the hybridization extension as found in the eye-field of the embryo un-injected side. βgal mRNA was visualized with Red-Gal. Image published in: De Marco N et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V.
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