XB-IMG-157143
Xenbase Image ID: 157143
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Fig. 4.
gipc2 rescues the eye-field phenotype of eif6-overexpressors, while gipc2 mut does not. a. In situ hybridizations with rax1 and pax6, in GFP, eif6 or eif6+gipc2 injected embryos. The arrows delimitate the site of the presumptive eye field in the side of the embryo where injection was performed. 1,4. The eye field in control GFP-injected embryos at neurula stage incubated with rax1 (1) or pax6 (4) riboprobe. 2,3. rax1 hybridization. 2. eif6-injected neurula shows that the eye field is smaller than in the counter-lateral eye, while the two eye fields have similar shape and extension in the eif6+gipc2-injected embryos (3). 5,6. pax hybridization. In eif6-injected neurula the eye field is smaller than in the counter-lateral eye (5), while in the eif6+gipc2-injected embryos the two eye fields have the same shape and extension (6). b. In situ hybridizations with rax1 of GFP and gipc2mut-injected embryos. Embryos injected with gipc2mut (2) indicate that rax1 riboprobe localization is partially reduced in the injected side, compared with the counter-lateral un-injected side, in contrast to the GFP-injected embryos (1). βgal mRNA was visualized with Red-Gal. c. The percentage of embryos with normal or reduced eyes is summarized. Image published in: De Marco N et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V.
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