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Figure 5. Usp7 is involved in recruitment of Dnmt1 to DNA replication sites in mammals. HeLa cells expressing shRNA-resistant wild-type Usp7(Wt) or its mutant (C223S) were infected with lentiviruses expressing tet-on shRNAs targeting Luciferase (Control) or Usp7 and treated with doxycycline (1 μg/ml) for 72 hrs. The resultant cells were subjected to immunostaining using anti-Dnmt1 (green) and anti-PCNA (red) antibodies.

Image published in: Yamaguchi L et al. (2017)

© The Author(s) 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license

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