Xenbase Image ID: 157278

Figure 2. Regulation of NPB cell fates by Bmp attenuation and Wnt signaling. Embryos at the two-cell stage were injected in the animal pole with increasing doses of Noggin (N) mRNA (0.1, 0.4, 1.0, and 2.0 ng) alone or in combination with Wnt1 (+Wnt) mRNA (100 pg). Animal explants were dissected at the blastula stage, cultured for 5 or 10 h, and analyzed by real-time RT-PCR for the expression of four putative NPB specifiers, Pax3, Zic1, Msx1, and Snail1 (A), and a number of cell type-specific genes: Snail2, Sox8, Twist, and cMyc for NC (B); Xhe and Crisp for HG (C); Six1 and Eya1 for PE (D); Sox2 and Ncam for neural plate (E); Xcg for cement gland (F); and Keratin for epidermis (G). Un, uninjected animal explant. Each value was normalized to the level of EF1α expression. Image published in: Hong CS and Saint-Jeannet JP (2007) Copyright © 2007. Image reproduced with permission of the Publisher, American Society for Cell Biology (ASCB). This is an Open Access article. Larger Image Printer Friendly View

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