Xenbase Image ID: 157281

Figure 5. Pax3 is sufficient and required to promote HG fate. (A) Embryos injected with Pax3GR and treated with dexamethasone at the gastrula stage hatch several hours earlier than their uninjected siblings. (B) Stage 17 embryo that received unilateral injection of Pax3GR at the two-cell stage and treated with dexamethasone (+Dex) exhibit a strong ectopic expression of Xhe on the injected side (right side), whereas sibling embryos cultured in the absence of dexamethasone (−Dex) are unaffected. Dorsal views, anterior to top. (C) In animal explants, Pax3GR strongly induces Xhe and Crisp expression 4 h after dexamethasone treatment (+Dex). Pax3-mediated induction of Xhe occurs independently of protein synthesis (+CHX). Another HG-specific gene, Crisp, is significantly reduced in the absence of protein synthesis. (D) Embryos injected with Pax3 morpholino (Pax3MO) exhibit a strong reduction of Snail2 and Xhe expression at the neurula and tailbud stages, respectively (arrows). Sox2 and Zic1 expression were expanded in Pax3-depleted embryos (arrows). Embryos hybridized with Snail2, Sox2, and Zic1 are viewed from the dorsal side, anterior to top. For Xhe staining, anterior view is shown. In all cases, the injected side is indicated by an arrow. (E) Noggin+Wnt activates expression of Snail2 and Xhe in animal explants, an activity that is inhibited by coinjection of Pax3MO. Image published in: Hong CS and Saint-Jeannet JP (2007) Copyright © 2007. Image reproduced with permission of the Publisher, American Society for Cell Biology (ASCB). This is an Open Access article. Larger Image Printer Friendly View

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