Xenbase Image ID: 157282

Figure 6. Distinct thresholds of Pax3 activity regulate HG and NC fates. (A) Embryos injected with increasing amounts of Pax3GR mRNA in one blastomere at the two-cell stage and treated with dexamethasone at the gastrula stage were analyzed by whole-mount in situ hybridization for Xhe and Snail2 expression at stage 17. Although Snail2 is expanded laterally and anteriorly by lower doses of Pax3GR, Snail2 is repressed for higher doses of Pax3GR. Ectopic expression of Xhe is proportional to the amount of Pax3GR mRNA injected. Dorsal views, anterior to top. In all panels, the injected side is on the right. (B) In animal explants, the induction of Snail2 and Xhe as determined by real-time RT-PCR depends on different amounts of injected Pax3GR. Zic1GR strongly synergizes with Pax3 to induce Snail2. The concentrations of Pax3GR mRNA injected are in nanograms. A single dose of Zic1GR mRNA (0.25 ng) was coinjected as indicated. Image published in: Hong CS and Saint-Jeannet JP (2007) Copyright © 2007. Image reproduced with permission of the Publisher, American Society for Cell Biology (ASCB). This is an Open Access article. Larger Image Printer Friendly View

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