Xenbase Image ID: 157283

Figure 7. Zic1 promotes the expression of Six1 an activity repressed by Pax3. (A) In animal explants, Zic1GR is sufficient to activate Six1 expression as determined by real-time RT-PCR. Pax3GR strongly represses Zic1-mediated Six1 induction to promote NC (Snail2) and HG (Xhe) fates. The concentrations of Zic1GR mRNA injected are in nanograms. A single dose of Pax3GR mRNA (0.25 ng) was coinjected as indicated. (B) Embryos injected with Zic1 morpholino (Zic1MO) exhibit a strong reduction of Snail2 and Six1 (arrows). The neural plate expression of Sox2 is expanded, whereas Sox2 PE expression domain is reduced (arrow). In these embryos lacking Zic1 function, Xhe expression is altered (arrow), presumably as a consequence of reduced Pax3 expression (arrow). Embryos are viewed from the dorsal side, anterior to top (Snail2 and Pax3). In Six1, Sox2, and Xhe staining, anterior views are shown, dorsal to top. The injected side is indicated by an arrow. (C) Noggin-mediated induction of Six1 is inhibited in the absence of Zic1 function (+Zic1MO). Noggin+Wnt activates expression of Snail2 and Xhe in animal explants, an activity that is inhibited by coinjection of Zic1MO. Image published in: Hong CS and Saint-Jeannet JP (2007) Copyright © 2007. Image reproduced with permission of the Publisher, American Society for Cell Biology (ASCB). This is an Open Access article. Larger Image Printer Friendly View

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