Xenbase Image ID: 157386

Figure 3. In Situ Hybridization to Normal, Dorsalized, and Ventralized Embryos Late blastula- and gastrula-stage embryos were hybridized with digoxigenin- labeled RNA probes. (A)-(G) show Xnr3 expression. (A) Vegetal view of late blastula-stage embryo (stage 9). Newly transcribed XnA message is restricted to nuclei on dorsal side of embryo. (6) Vegetal view of early gastrula-stage embryo (stage 10). Arrow marks the dorsal lip of the blastopore. Unlike other organizer-specific genes, hybridizing cells can be found on both sides of the blastopore lip. (C) Vegetal view of midgastrula embryo (stages 10.5-l 1). Hybridization signal is significantly reduced from early gastrula stage and is restricted to cells around the blastopore. (D) Animal pole view of dorsalized (Lit-treated) embryo at the late blastula stage (stage 9). (E) Side view of embryo in (D). (F) Vegetal pole view of ventralized (UV-treated) embryo at the late blastula stage. No specific XnA hybridization could be detected. (G) Sagittal section of stage 9 embryo through the dorsal-ventral axis. XnA hybridization is found only in the epithelial layer of the organizer. (H) Section as in (G), but with noggin hybridization probe. Hybridization is detected in both the epithelial and deep layers of the organizer. (I) Section as in (G), but with goosecoid hybridization probe. Hybridization is excluded from the epithelial layer of the organizer. The outer edge of the epithelial layer is marked by the broken line. (J) Section as in(G), but with Erachyury hybridization probe. Hybridization is similar to that seen with noggin, with the exception that it is found on both dorsal and ventral sides of the embryo. Image published in: Smith WC et al. (1995) Copyright © 1995. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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