XB-IMG-157425
Xenbase Image ID: 157425
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Figure 1—figure supplement 1.
Pent constructs are biologically active in Drosophila, but XSMOC-1 protein is not detected in Drosophila following mRNA overexpression.
(A–C) Adult Drosophila longitudinal wing vein (L1 to L5) patterns in (A) wild type; arrow indicates the position of the anterior crossvein between L4 and L5. (B) pent2-5/pentA17 mutant lacking pent where L5 is missing (C) Rescue of pent2-5/pentA17 phenotype by expression of Pent in a pent2-5/pentA17 background using the UAS/Gal4 system with brinker (brk)/Gal4 as the driver. (D) Immunoblot of Drosophila embryo extracts (10 μg/lane) from control yellow white (YW) Drosophila embryos spiked with XSMOC-1 (Lanes 1–5) as indicated, XSMOC-1 only (Lane 6), or Drosophila lines 4 (Lane 7) and 5 (Lane 8) overexpressing XSMOC-1 mRNA. (E) Coomasie-stained SDS-PAGE gel following protein transfer shown in (D), showing residual protein. The amount of protein in lane seven is notably more than that in other lanes, but the XSMOC-1 signal is nevertheless undetectable.
DOI: http://dx.doi.org/10.7554/eLife.17935.004 Image published in: Thomas JT et al. (2017) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View |