XB-IMG-157428
Xenbase Image ID: 157428
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Figure 2—figure supplement 1.
Dimeric XSMOC-1 is not dissociated by chelation or reduction.
(A) SEC profile showing that XSMOC-1, refolded in the presence of 2 mM CaCl2, continues to migrate as a dimer (↓) upon the subsequent chelation of calcium ions by dialysis in the presence of 10.5 mM EDTA. (B) SDS-PAGE analysis of XSMOC-1, XSMOC-1 ∆EC, and XSMOC-1 EC in the presence (+) and absence (−) of β-mercaptoethanol shows that the XSMOC-1, XSMOC-1 ∆EC dimers are not linked by disulfide bonds.
DOI: http://dx.doi.org/10.7554/eLife.17935.007 Image published in: Thomas JT et al. (2017) Image downloaded from an Open Access article in PubMed Central. Image reproduced on Xenbase with permission of the publisher and the copyright holder. Larger Image Printer Friendly View |