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Fig 4. Brg1 protein knockdown diminishes gene expression in the BCNE center. Pictures show dorsal views of whole mount RNA in situ hybridization (WMISH) at late blastulae (NF9, X. laevis) with gene expression patterns representative for the experimental condition. Injection conditions are indicated on top: CoMO (60ng/embryo), BMO1 (60ng/embryo), Rescue (BMO1 plus hBRG1 mRNA [1ng/embryo]). Reagents were radially injected into the animal hemisphere. The mRNA expression patterns of chordin, noggin and nodal-related 3 (nr3) in control morphants (panels A-C) were reduced in BMO1 morphants (panels A’–C’), but restored by coinjection of human brg1 mRNA (panels A” –C”). The graphs (A”‘–C”‘) depict phenotypic penetrance (n = 3–4 experiments/condition). The expression of siamois was not affected by BMO1 (panels D + D’, n = 2 experiments/gene). Image published in: Wagner G et al. (2017) © 2017 Wagner et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue chrd.L chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Throughout NF stage 9 dorsal marginal zone nog.L nog-A, nog1, noggin, noggin 1, noggin-1, Noggin1 X. laevis Throughout NF stage 9 dorsal marginal zone nodal3.1.L nr3, Xnr-3, Xnr3 X. laevis Throughout NF stage 9 dorsal marginal zone sia1.L sia, Siamois, Xsia X. laevis Throughout NF stage 9 dorsal marginal zone Image source: Published Larger Image Printer Friendly View

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