Xenbase Image ID: 157526

Supp F3. Functional annotation and validation of BMO1 responding genes. Panels (A, B) show the top five GO-terms for up- or downregulated genes in X. tropicalis BMO1 morphants. Panels (C, D)—qRT/PCR based verification of microarray data in radially injected X. tropicalis embryos (n = 5–8 independent experiments/gene). Noggin was included based on its function as neural inducer, even though the microarray contained no probeset for this gene. Asterisks mark genes, which were significantly downregulated in the qRT/PCR analysis. Panel (C) Blastula signaling centers. Chordin, noggin, follistatin and bmp4 mRNAs were downregulated. Panel (D) shows germ layers markers: zic1, sox21 and gata4 were downregulated, while the upregulation of egr1 was not statistically verified. RNA in situ analysis of radially injected X. tropicalis morphant blastulae: foxd4l1 (E, F), noggin (G, H) and zic2 (I, K). (E’-K’) show corresponding sagittal sections. Whereas foxd4l1 and noggin are downregulated in BMO1 morphants, zic2 gene expression is not changed (n = two biological replicates). Image published in: Wagner G et al. (2017) © 2017 Wagner et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Gene Synonyms Species Stage(s) Tissue foxd4l1.1.L foxd4, Foxd4l1, foxd4l1.1-a, foxd4l1.1-b, foxd5, foxD5a, FoxD5b, Xfd-12, XFlip, xlFoxD5a, xlFoxD5b, xtFoxD5 X. laevis Throughout NF stage 11 mesoderm blastocoel roof nog.L nog-A, nog1, noggin, noggin 1, noggin-1, Noggin1 X. laevis Throughout NF stage 11 dorsal marginal zone zic2.L hpe5, xzic2, zic2-a, zic2-b, Zinc finger protein of the cerebellum 2 X. laevis Throughout NF stage 11 marginal zone Image source: Published Larger Image Printer Friendly View

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