Xenbase Image ID: 157527

Supp F4. Dose-dependent consequences of systemic BMO1 depletion in X. laevis. BMO1 morpholinos were injected four times into the animal pole region at the two- to four-cell stage (“radial” injection type). Panel (A): Typical morphology of embryos injected with increasing dose of BMO1. UI–uninjected siblings. BRG1 depleted embryos get arrested in gastrulation. Top rows: brightfield image; bottom row: green fluorescence from coinjected Alexa488 dextran. Panel (B) shows representative images of embryos that have survived until heartbeat stage (NF34). While most embryos are still arrested in gastrulation, some embryos injected with the lowest BMO1 dose (20 ng) have completed gastrulation but are arrested at open neural plate stage. Axial structures and closed neural plates were never observed. Panel (C) gives quantification of the BMO1 titration (n = 3–5 biological replicates/condition). Image published in: Wagner G et al. (2017) © 2017 Wagner et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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