Xenbase Image ID: 157533

Supp F10. Brg1 is required for the transcriptional burst at the MBT. Panel (A) displays experimental scheme of sample collection for genome-wide comparison of preMBT versus postMBT transcriptomes (X. tropicalis). (B) The preMBT sample closest to the MBT (see sample #3 in red) was identified by qRT/PCR analysis for the marker gene gs17. Gs17 mRNA levels of the “early” samples 1–5 were normalized to the value of four cell stage embryos (n = 3 biological replicates). The postMBT sample (here #2 in red) was chosen as the one being harvested 40 min before the appearance of the blastoporus pigmentation lip in the sibling cohorts. This time point correlates with the late blastula stage used for the BMO1 microarray analysis of Fig 1. (C) The top 4 enriched GO-terms in the class of highly upregulated genes at MBT. (D) Heatmap providing mean expression levels at the pre- and postMBT timepoints for genes of the GO-term “nervous system development”, ranked by amplitude of mRNA increase (log2 fold-change). Genes that were downregulated in the BMO1-morphant transcriptome, compared to the control morphant state are marked with an asterisk. Like in the term “pattern specification process” only genes that show a marked upregulation of mRNA after MBT were affected by the Brg1 protein knockdown. Image published in: Wagner G et al. (2017) © 2017 Wagner et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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