Xenbase Image ID: 157587

Figure 1 Phosphorylation of Akt in response to ionizing radiation. (A) Embryos were not irradiated (control) or were exposed to γ-IR at either stage 6 or stage 9, collected at different times, and analyzed as described in Materials and Methods. The blots were incubated with anti-P-Ser 473 Akt antibody (Upper) or anti-Akt antibody (Lower) and visualized by enhanced chemiluminescence. The arrows on the right denote the Akt protein and the phosphorylated isoforms. Molecular mass markers (in kDa) are indicated on the left. (B) (Upper) Embryos were injected at the one-cell stage with β-galactosidase, WT-, DN-, or CA-Akt mRNA and allowed to develop until they reached stage 6 (Top row). At that stage, embryos were not irradiated (first column on left) or were treated with ionizing radiation (γ-IR) and allowed to progress through early developmental stages. Embryos were collected and photographed at the time of irradiation (Top row, stage 6), at the MBT (Middle row, stage 8), and 7 h after the MBT (Bottom row), when the morphological changes of apoptosis were conspicuous (scale bar, 1 mm). (Lower) At the indicated stages, levels of the indicated expressed proteins were analyzed by Western blotting. Image published in: Finkielstein CV et al. (2001) Copyright © 2001. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page