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Figure 3 (A) Analysis of the level of p27Xic1 in embryos irradiated either pre- or post-MBT. Embryos were not irradiated (control) or were irradiated (γ-IR) at the indicated stages, and samples were collected at the indicated times and analyzed by Western blotting with anti-p27Xic1 antibody. One embryo equivalent was loaded per lane. (B) Cell cycle profile of irradiated embryos. Embryos were irradiated at stage 9 and collected 2 and 6 h after irradiation. Embryonic nuclei were prepared from a single sibling group and analyzed by flow cytometry as described in Materials and Methods. Stages of the cell cycle are defined by DNA content, as measured by fluorescence intensity.

Image published in: Finkielstein CV et al. (2001)

Copyright © 2001. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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