Xenbase Image ID: 157590

Figure 4 Targets for p27Xic1 in vivo. Embryos were not irradiated (control) or were irradiated after the MBT (stage 9 γ-IR), collected at different times, and frozen. (A) The total level of p27Xic1 at the indicated times. (B) Samples equivalent to five embryos were precipitated with p13suc-1 beads, and the bound proteins were analyzed by Western blotting with anti-p27Xic1 antibody. (C) Association of p27Xic1 with cyclin A2 complexes was detected by immunoprecipitation with cyclin A2 antibody and analysis of the precipitates by Western blotting with anti-p27Xic1 antibody. (D) Samples from embryos irradiated after the MBT were immunoprecipitated with anti-Cdk4 antibody and blotted with anti-p27Xic1 antibody. Image published in: Finkielstein CV et al. (2001) Copyright © 2001. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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