XB-IMG-157639
Xenbase Image ID: 157639
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Fig. 2. Axis duplication by dkk2. (a) Expression of Xdkk2 determined by in situ hybridisation. Lateral view of a stage 40 Xenopus embryo showing expression of dkk2 in head mesenchyme (hm), somites (so), and lens (le). (b,c) Injected dkk2 RNA induces (b) incomplete and (c) complete secondary embryonic axes. Mouse dkk2 RNA (50–250pg) was injected into one ventral blastomere at the 8-cell stage. (d,e) Injected dkk2 RNA induces siamois expression. Embryos (4–8-cell stage) were uninjected (Co) or injected in the animal region with 500pg Xdkk1, or Xenopus or mouse dkk2 RNA. Animal caps were explanted at stage 8 and analysed at (d) stage 11 or (e) stage 30 by RT–PCR analysis for the expression of the indicated marker genes. (f,g) Injected dkk2 RNA activates a TCF-responsive promoter; 50pg pTOP-luc and 5pg pTK-renilla were coinjected either alone (Co) or with the indicated RNAs (Xwnt8, 25pg; Xfz8, 200pg; mdkk2 or mdkk3, 100pg; GSK-3, 1.5ng). Luciferase activity was determined and normalised against renilla activity. (h) Injected dkk2 RNA cooperates with fz2, fz5, and fz8. Expression of siamois in animal caps injected with 25 pg Xwnt8, or RNAs encoding the indicated Fz receptors (Dfz2, 100pg; Xfz8, 200pg; all others, 500pg) injected with or without 100pg mdkk2 RNA as indicated. Image published in: Wu W et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V.
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