Xenbase Image ID: 157670

Fig. 2. BMP signaling is required for definitive and primitive hematopoiesis in Xenopus laevis. (A) Noggin protein induced by heat-shock treatment at stage 14 leads to a complete loss of tal1 and hba3 expression in ventral blood islands (arrows) in HS:Noggin transgenic Xenopus stage 32 embryos. Similarly, DMH1 treatment starting from stage 14 blocks tal1 and hba3 expression. (B) Inhibition of BMP signaling by DMH1 from stage 14 abrogates expression of myeloid blood markers mpo and spib at stage 33 compared with DMSO-treated control. (C and D) Inhibition of BMP signaling from stage 14 by Noggin (C) or DMH1 (D) results in the loss of expression of hemogenic endothelium markers runx1, spib, and gfi1a in the DA (red arrow for absence of in treated, green arrows for normal expression in control embryos). (E and F) The endothelium marker pecam1 and arterial gene dll4 is expressed in control as well as treated samples, albeit slightly lower in HS:Noggin embryos. The arterial marker efnb2 is severely reduced in either HS:Noggin-expressing or DMH1-treated embryos (red arrows in E and F). The stages at which the in situ hybridization was performed are indicated on the top left corner. Embryos were photographed from a lateral view, with anterior on the left and dorsal at the top. Numbers in bottom right corner indicate proportion of embryos displaying the phenotype. (Scale bars: 0.5 mm.) Image published in: Kirmizitas A et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue hba3.L alpha globin, alpha-globin, globin alpha T3, globin T3, Ta-globin X. laevis Throughout NF stage 32 ventral blood island hematopoietic stem cell ventral blood island border posterior ventral blood island erythroid cell tal1.L bHLHa17, stem cell leukaemia, tal-1, tcl5, xscl X. laevis Throughout NF stage 32 ventral blood island hematopoietic stem cell ventral blood island border posterior ventral blood island erythroid cell mpo.L mpo-A, myeloperoxidase, pmr-1, pmr1, pox2, xpox2 X. laevis Throughout NF stage 32 myeloid cell hematopoietic stem cell spib.L spi-b, spib-a, spib-b X. laevis Throughout NF stage 32 ventral blood island hematopoietic stem cell ventral blood island border posterior ventral blood island myeloid cell runx1.L aml, aml-1, aml1, aml1 oncogene, aml1-evi-1, amlcr1, cbfa2, evi-1, LOC108709238, pebp2ab, Runx-1, Xaml, Xaml1 X. laevis Throughout NF stage 39 endothelial cell vascular endothelium spib.L spi-b, spib-a, spib-b X. laevis Throughout NF stage 39 endothelial cell vascular endothelium gfi1.L gfi1a X. laevis Throughout NF stage 39 endothelial cell vascular endothelium pecam1.L cd31, pecam, pecam-1 X. laevis Throughout NF stage 37 and 38 endothelial cell vascular endothelium arterial endothelium intersomitic vessel intersomitic artery dll4.L delta 2, LOC108705808 X. laevis Throughout NF stage 37 and 38 endothelial cell vascular endothelium arterial endothelium intersomitic vessel intersomitic artery efnb2.L efnb2-a, efnb2-b, ephrin B2, ephrinB2, Xefnb2 X. laevis Throughout NF stage 37 and 38 endothelial cell vascular endothelium arterial endothelium intersomitic vessel intersomitic artery blood vessel vascular plexus Image source: Published Larger Image Printer Friendly View

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