Xenbase Image ID: 157674

Fig. 4. BMP signaling is required for gata2 expression in the DLP transcriptional network. (A) Hematopoietic programming in the DLP, at stage 25, following heat shock-induced noggin expression at stage 15. Embryos were heat-shocked for 15 min at 35 °C at stage 15 and were collected for analysis at stage 25. Fli1 expression is unaffected in the DLP (green arrows), whereas gata2, kdr, tal1, and lmo2 expression is absent in the DLP as well as ventral blood island (VBI) (red arrows) of HS:Noggin transgenic siblings. Etv2 expression is decreased in the DLP and is absent in VBIs (red arrow). Numbers on individual images show the proportion of embryos for which that image represents (bottom right). (B) qPCR analysis of hemangioblast genes in stage 25 DLPs dissected from control and heat-shock noggin transgenic embryos. Fli1 is not significantly affected, whereas all of the other genes quantified show significant decreases of expression. (C) Hematopoietic programming in the DLP, at stage 26, following heat shock-induced noggin expression at stage 20. The expression of fli1, gata2, etv2, and lmo2 is unaffected. The expression of kdr and tal1 is reduced in the DLP hemangioblasts. HS:Noggin transgenic siblings were identified by the expression of Noggin transcripts (uniform Fast Red staining in HS:Noggin embryos). (D) Gata2 expression in the DA, at stage 36, following heat shock-induced noggin misexpression at stages 23 and 28. Gata2 expression is absent in the DA of HS:Noggin transgenic siblings when heat shock is done at stage 23 (red arrow), whereas gata2 is expressed in the DA of HS:Noggin siblings when heat shock is done at stage 28, compared with wild-type siblings (green arrows). The stage of the embryos are indicated in the top left corner of the top left image. Numbers on individual images shows the proportion of embryos that the image represents (Bottom Right). Images and numbers are from one experiment and are representative of two biological replicates. (Scale bars: 0.5 mm.) Image published in: Kirmizitas A et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue fli1.S fli, fli-1, fli1-a, Xfli, Xfli-1, Xfli1 X. laevis Throughout NF stage 26 neural crest cranial neural crest premigratory neural crest cell dorsal lateral plate mesoderm anterior dorsal lateral plate region hematopoietic stem cell lateral plate mesoderm ventral mesoderm gata2.L GATA-2, xGATA-2, xgata2 X. laevis Throughout NF stage 26 hindbrain spinal cord dorsal lateral plate mesoderm anterior dorsal lateral plate region ventral blood island hematopoietic stem cell posterior ventral blood island lateral plate mesoderm ventral mesoderm etv2.L er71, ets1, ets2-like, Etsrp, etsrp71 X. laevis Throughout NF stage 26 dorsal lateral plate mesoderm anterior dorsal lateral plate region hematopoietic stem cell lateral plate mesoderm ventral mesoderm prechordal plate kdr.L flk-1, flk1, LOC108698463, vegfr-2, vegfr2, xflk-1 X. laevis Throughout NF stage 26 prechordal plate neural crest cranial neural crest lateral plate mesoderm ventral mesoderm dorsal lateral plate mesoderm anterior dorsal lateral plate region ventral blood island hematopoietic stem cell posterior ventral blood island tal1.L bHLHa17, stem cell leukaemia, tal-1, tcl5, xscl X. laevis Throughout NF stage 26 lateral plate mesoderm anterior dorsal lateral plate region ventral mesoderm dorsal lateral plate mesoderm spinal cord prechordal plate midbrain-hindbrain boundary lmo2.L LMO-2, xlmo-2, xlmo2 X. laevis Throughout NF stage 26 lateral plate mesoderm anterior dorsal lateral plate region ventral mesoderm ventral blood island chordoneural hinge presomitic mesoderm caudal presomitic mesoderm posterior ventral blood island gata2.L GATA-2, xGATA-2, xgata2 X. laevis Throughout NF stage 35 and 36 dorsal aorta intersomitic vessel blood vessel vascular plexus lateral plate mesoderm dorsal lateral plate mesoderm Image source: Published Larger Image Printer Friendly View

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