XB-IMG-157687
Xenbase Image ID: 157687
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Figure 2 Phase contrast image of tissue cultured Xenopus RPE1choroid on Day 14 and (A to H)
detection of Xmmp9 and Xmmp18 expression on Day 5. Normally, 4 different zones can be discriminated
around Day 14 of culture, depending on the cell–cell interactions. In the epithelial zone, both
N-cadherin and Connexin-43 are detected, while in the transitional zone only N-cadherin can be
detected (Nabeshima et al., 2013). (A–D) In situ hybridization of Xmmp9 and Xmmp18 at the early
stage of culture. RPE cells migrating from the periphery of the explant (asterisks) and in the epithelial
zone are positively stained for both Xmmp9 and Xmmp18. (E–H) No positive staining was
detected when sense probes were applied. Cultures were treated for de-pigmentation before they
were processed for in situ hybridization. [Color figure can be viewed at wileyonlinelibrary.com] Image published in: Naitoh H et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |