Xenbase Image ID: 157690

Figure 5 Effects of 1, 10-PNTL on RPE cell proliferation. (A,B) In the control cultures, numerous cells in the transitional zone were labeled for BrdU. (C) By applying double immunostaining for BrdU (red color) and connexin-43 (green color), RPE cells that were labeled for BrdU were observed mostly in the transitional zone, while in the epithelial zone cells were stained for connexin-43 and were not labeled for BrdU. (D, E) In the presence of the inhibitor, only a few cells were labeled for BrdU. Asterisks indicate tissue explants and the dotted lines indicate the boundaries between the explant and the epithelial zone. Asterisks indicate tissue explants and the dotted lines indicate the boundaries between the explant and the epithelial zone. (F) Quantification of labeled cells for BrdU indicates that the inhibitor substantially inhibited RPE cell proliferation. The data are the mean cell number from four explants at each concentration of the inhibitor. For details of cell counting, see the methods section. Three separate experiments were performed and the representative data are shown in the figures. *, p<0.05 vs. cultured without the inhibitor. [Color figure can be viewed at wileyonlinelibrary.com] Image published in: Naitoh H et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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