XB-IMG-157692
Xenbase Image ID: 157692
|
Figure 7 Effects of the anti-inflammatory drugs dexamethasone (Dex) and Withaferin A on RPE cell
migration and neuronal differentiation. (A–C) On Day 12 in the culture lacking Dex, RPE cells had
migrated out from the periphery of the explant. The arrow indicates the distance between the periphery
of the explant and the most outward RPE cells. Cells did not actively migrate when Dex was present
in the culture at 50 mM (B) or 100 mM (C). (D, E) Similar effects by Withaferin A were observed on
Day 8, and Withaferin A mostly suppressed RPE cell migration at 10 mM. Asterisks indicate tissue
explants. (F) When neuronal differentiation was detected with ßIII tubulin immunostaining, Dex suppressed
differentiation of ßIII tubulin-positive cells in a Dex-concentration-dependent manner. The
data are the mean cell numbers from four explants at each concentration of the inhibitor. The results
represent data from three separate experiments. *, p<0.05; **, p<0.01 vs. cultured without Dex.
[Color figure can be viewed at wileyonlinelibrary.com] Image published in: Naitoh H et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |