Xenbase Image ID: 157698

Fig. 2. Analysis of transcripts in embryos targeted for MyoD using CRISPR/Cas9. Experimental and control embryos were collected at NF stage 11.5. (A) qPCR analysis shows the expression of MyoD and the known MyoD target gene Rbm24. Pair-wise t-tests for the mean relative expression for Cas9 only and Cas9 plus gRNA injected sets for each gene. Error bars represent SEM, * = P < 0.05, ** = P < 0.01. (B) MyoD sequences returned from mapping raw RNA-Seq reads to the Xenopus tropicalis myod1 gene. A total of 1200 reads were extrapolated across the three biological replicates and the proportion of reads showing each mutation type was calculated. The mutated sequences are shown aligned to the sequence for wild type MyoD. The total number of reads for each of the mutations shown is as follows: #1 = 175 (15%), #2 = 13.5 (1%), #3 = 219 (18%), #4 = 70 (6%), #5 = 69.5 (6%), #6 = 40 (3%). Image published in: McQueen C and Pownall ME (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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