Xenbase Image ID: 157699

Fig. 3. Overview of results from RNA-Seq. RNA-Seq reads were mapped using the Xenopus tropicalis genome version 9.0 (Xenbase). FPKM (fragments per kilobase of transcript per million mapped reads) values were calculated to avoid bias towards longer genes by normalising the number of reads per fragment to the length. FPKM values for three biological replicates were analysed by pairwise t-tests comparing expression in Cas9 only control embryos and MyoD CRISPR-targeted samples. A volcano plot showing significance value vs fold change was constructed in Python. Genes in blue indicate a fold change of > 1, genes in yellow indicate a fold change > 1 and a P value of < 0.05. Image published in: McQueen C and Pownall ME (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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