Xenbase Image ID: 157701

Fig. 5. Validation of a set of candidate target genes that require MyoD for expression at NF St11.5. qRT-PCR analysis of identified putative early gene targets of MyoD. Analysis shows the expression of MyoD and the known MyoD target gene Rbm24 alongside predicted target genes Rbm20, Gli2, Foxc1 and Zeb2. Pair-wise t-tests were carried out for the mean relative expression of three biological replicates for Cas9 only and Cas9 plus gRNA injected sets for each gene. Error bars represent SEM, and * = P < 0.05, ** = P < 0.01. Image published in: McQueen C and Pownall ME (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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