XB-IMG-157703
Xenbase Image ID: 157703
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Fig 2. Double colour 5S and 28S rDNA FISH on X. tropicalis and X. mellotropicalis chromosomes.
DAPI counter-stained metaphase spreads showed all (A) 20 chromosomes (B&W) in X. tropicalis and/or (D) 40 chromosomes in X. mellotropicalis. 28S rDNA amplicon labelled by Digoxigenin-11-dUTP (green) stained (B) XTR 7 secondary constriction and telomeres of XTR 6, 7 and 9 and/or (E) XME 7a secondary constriction and telomeres of XME 4a and 5b. 5S rDNA amplicon labelled by Biotin-16-dUTP (red) revealed positive signals on telomeric segments of (C) XTR 2, 3, 4, 5, 6, 7, 8 and 9 and/or (F) XME 4a, 5b and 8b. Arrows show NORs situated on secondary constrictions of (B) XTR 7 and (E) XME 7a. Scale bar represents 10 μm.
https://doi.org/10.1371/journal.pone.0177087.g002 Image published in: Knytl M et al. (2017) © 2017 Knytl et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |