Xenbase Image ID: 157781

Figure 2 Transactivation potential of the P328L329 mutant. (a) Increasing amounts of expression vector encoding P328L329 or HNF1β were cotransfected into HeLa cells with a luciferase reporter containing four HNF1-binding sites (23). The fold activation compared with transfections lacking any transfected factor is given. The SDs from at least six determinations are presented, and the methods used have been described (23). (b) RNA encoding HNF1α, HNF1β, or P328L329 was injected into fertilized Xenopus eggs, and the RNA was extracted at the late gastrulae stage (stage 12.5). Endogenous HNF1α and ODC (ornithine desoxycarboxylase) transcripts were determined by reverse transcription–PCR as described (24). The control is RNA of a stage 20 embryo. Stages are as given in ref. 25. Image published in: Wild W et al. (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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